产品内容

使用注意事项

1. 在配制完全培养基前，请把胎牛血清（FBS）、间充质干细胞生长添加剂(MSCGS)、青霉素-链霉素(P/S)放到2-8℃冰箱过夜解冻，然后加入到基础培养基中。

2. 在使用完全培养基前，需要把培养基放到37℃恒温水浴中预热，注意温度不要超过37℃。

3. 请把配制好的完全培养基放在4℃冰箱避光保存，并尽量在一个月内使用完。避免反复冻融，若培养基要分几次使用，请将胎牛血清（FBS）、间充质干细胞生长添加剂(MSCGS)、青霉素-链霉素(P/S)解冻按用量分装后保存。

产品用途

仅供研究使用。不适用于人或动物体外诊断与治疗。

培养条件

37℃，5% CO2，无菌恒温培养。

• 人脂肪间充质干细胞培养基说明书查看>>

1. Injectable microcryogels reinforced alginate encapsulation of mesenchymal stromal cells for leak-proof delivery and alleviation of canine disc degeneration.
Zeng Y1, Chen C2, Liu W1, Fu Q3, Han Z4, Li Y1, Feng S3, Li X1, Qi C1, Wu J4, Wang D4, Corbett C5, Chan BP6, Ruan D7, Du Y8.
1.Department of Biomedical Engineering, School of Medicine, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Tsinghua University, Beijing 100084, China.
2.Department of Orthopaedics, Navy General Hospital, Beijing 100048, China; Department of Orthopedic Surgery, First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
3.School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
4.Department of Orthopaedics, Navy General Hospital, Beijing 100048, China.
5.Department of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD 21231, USA.
6.Tissue Engineering Laboratory, Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Rd, Hong Kong Special Administrative Region, China.
7.Department of Orthopaedics, Navy General Hospital, Beijing 100048, China. Electronic address: ruandikengh@163.com.
8.Department of Biomedical Engineering, School of Medicine, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Tsinghua University, Beijing 100084, China. Electronic address: duyanan@tsinghua.edu.cn.
【Human MSCs or Canine MSCs were then suspended at a density of 2×106 in 10ml 5% FBS contained MSC basal medium (Biowit, China) and cultures at 37℃,5% CO2. 】

2. Human adipose tissue_derived stem cells alleviate radiation_induced xerostomia.
XUEYAN XIONG1*, XIUJUAN SHI2* and FENGSHAN CHEN1
1.Laboratory of Oral Biomedical Science and Translational Medicine, Department of Orthodontics, School of Stomatology, Tongji University, Shanghai 200072;
2.School of Medicine, Tongji University, Shanghai 200092, P.R. China
【The medium was changed to mesenchymal stem cell medium(MSCM; BioWit Technologies, Shenzhen, China) 8h after infection. 】

3. Magnetically Controllable 3D Microtissues based on Magnetic microcryogels.
Liu W1, Li Y, Feng S, Ning J, Wang J, Gou M, Chen H, Xu F, Du Y.
Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, PR China. duyanan@tsinghua.edu.cn.
【Human adipose-derived mesenchymal stromal cells (hMSCs) were isolated and cultured in the mesenchymal stem cell growth medium (BioWit Technologies)】

4. Synthesis and properties of poly (3-hydroxybutyrate-co-3-hydroxyvalerate)/chitin nanocrystals composite scaffolds for tissue engineering.
Wang B1, Li J, Zhang J, Li H, Chen P, Gu Q, Wang Z.
Ningbo Institute of Material Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, PR China
【hADSC-GM (human adipose-derived stem cell growthmedium) were purchased fromBiowit Technologies (Shenzhen, China) 】

5. 利用细胞外基质大规模扩增临床级人脂肪间充质干细胞.
苏约翰1，卫 超2，吕品雷2，曹 蕴1，邱 云2，郑 青2，萧树东2，汪 铮1，2
1解放军第455医院，解放军南京军区生物细胞工程技术医学转化基地干细胞实验室，上海市 200052；
2上海交通大学医学院附属仁济医院消化科，上海市消化疾病研究所干细胞实验室，上海市 200001)
【脂肪间充质干细胞培养液 Biowit Technologies 公司】

6. 荧光磁性纳米粒子标记三维培养系统中的脂肪间充质干细胞
吕品雷1，苏约翰1，崔大祥2，汪 铮1
(1上海交通大学医学院附属仁济医院消化疾病研究所，上海市 200001；2上海交通大学微纳科学
技术研究院纳米生物工程研究室，上海市 200240)
【脂肪间充质干细胞培养液 深圳百恩维生物科技有限公司】