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使用百恩维生物腺相关病毒发表的文章

2016-02-16

1.Overexpression of 1 ankyrin repeat domain 1 enhances cardiomyocyte apoptosis by promoting p53 activation and mitochondrial dysfunction in rodents
Liang SHEN*#, Ci CHEN*#, Xuan WEI*, Xixian LI*, Guangjin LUO*, Jingwen ZHANG *, Jianping BIN*, Xiaobo HUANG*, Shiping CAO*, Guofeng LI†, Yulin LIAO*
*State Key Laboratory of Organ Failure Research,Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
†Department of Pharmacy, Nanfang Hospital, Southern Medical University,Guangzhou 510515, China
# These authors contributed equally to this work.
【pAAV2/9-CMV-ZsGreen (AAV: adeno-associate virus) 34 vectors carrying sh-Ankrd1 (short hairpin of Ankrd1) or negative control were generated by a 35 professional company (Biowit, Shenzheng, China). 】


2.Treatment of rabbit intervertebral disc degeneration with co-transfection by adeno-associated virus-mediated SOX9 and osteogenic protein-1 double genes in vivo
SHAN REN, YONGJUN LIU, JINFENG MA, YONG LIU, ZEZHENG DIAO, DELING YANG, XU ZHANG, YONGMING XI and YOUGU HU
Department of Spine Surgery, Affiliated Hospital of Medical College,Qingdao University, Qingdao, Shandong 266003, P.R. China
【Recombinant AAV (rAAV)-OP1, rAAV-SOX9 and rAAV-EGFP were provided by Biowit Technologies Co., Ltd. (Shenzhen, China). 】

 

3. VEGF-Mediated Proliferation of Human Adipose Tissue-Derived Stem Cells
Guangfeng Chen1., Xiujuan Shi1., Chen Sun1, Min Li2, Qing Zhou2, Chen Zhang1, Jun Huang1, Yu Qiu1,
Xiangyi Wen1, Yan Zhang1, Yushan Zhang1, Shuzhang Yang1, Lixia Lu1, Jieping Zhang1, Qionglan Yuan1,
Jianwei Lu1, Guotong Xu1, Yunyun Xue1, Zibing Jin3, Cizhong Jiang2*, Ming Ying1,4*, Xiaoqing Liu1,4*
 (1 Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, China, 2 Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China, 3 Division of Ophthalmic Genetics, The Eye Hospital of Wenzhou Medical College and Lab for Stem Cell & Retinal Regeneration, School of Ophthalmology & Optometry, Wenzhou Medical College, Wenzhou, China, 4 Shenzhen Key Laboratory of Marine Bioresources and Ecology,College of Life Sciences, Shenzhen University, Shenzhen, China)
【Short hairpin RNA (shRNA) for the VEGF gene silencing in an adeno-associated viral vector (AAV) were constructed and packaged into viral particles with a titer of over 161012/ml by BioWit Technologies Ltd. Lentiviral vector harboring microRNAs were constructed into pLVX-ShRNA2 vector and particles were produced by using 2-generation packaging mix (BioWit Technologies Ltd.) with over a titer of over 16108 TU.】
【A lentiviral vector with a putative microRNA 21-recognition element (single copy) from the VEGF gene fused with the firefly luciferase gene as a 39-untranslated region was provided by BioWit Technologies Ltd.】
【The cell pellet was resuspended in DMEM/F12 medium (GIBCO) supplemented with 10% FBS (GIBCO), 10%KSR (Invitrogen) or ADSC serum-free media (Biowit Technologies Ltd, Quincy, USA) at a density of 26106 cell/mL.】

 

4. Cytosolic CARP Promotes Angiotensin II- or PressureOverload-Induced Cardiomyocyte Hypertrophy through
Calcineurin Accumulation
Ci Chen1, Liang Shen1, Shiping Cao1*, Xixian Li1, Wanling Xuan1, Jingwen Zhang1, Xiaobo Huang1,Jianping Bin1, Dingli Xu1, Guofeng Li2, Masafumi Kitakaze1,3, Yulin Liao1*
1 State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou, China,
2 Department ofPharmacy, Nanfang Hospital, Southern Medical University, Guangzhou, China,
3 Cardiovascular Division of the Department of Medicine, National Cerebral and Cardiovascular Center, Osaka, Japan
【pAAV2/9-CMV-ZsGreen vectors carrying short hairpin RNA targetingAnkrd1 (sh-Ankrd1) or scramble were created by a professional company (Biowit, Shenzheng, China).】


5. Regulation of RyR2 Channels of Mongrel canines with Heart Failure by Means of FKBP12.6 Gene
Juan Sun, Yanmei Lu, Yan Huang, Ling Zhang, Yitong Ma
Ma/Exp Clin Cardiol Vol 20 Issue8 pages 2792-2811 / 2014
【AAV9/EGFP and AAV9/FKBP12.6-EGFP were purchased from Shenzheng BioWit Technologies Co., Ltd and its titer were1.0×1012vg/ml.】


6. miR-29a-3p Attenuates Hypoxic Pulmonary Hypertension by Inhibiting Pulmonary Adventitial Fibroblast Activation
Ying Luo, Hai-Ying Dong, Bo Zhang, Zhao Feng, Yi Liu, Yu-Qi Gao, Ming-Qing Dong and Zhi-Chao Li
Hypertension. published online November 24, 2014;
Hypertension is published by the American Heart Association, 7272 Greenville Avenue, Dallas, TX 75231 Copyright © 2014 American Heart Association, Inc. All rights reserved.
Print ISSN: 0194-911X. Online ISSN: 1524-4563

【annealed and cloned into pAAV-ZsGreen-miRNA vector (Biowit Technologies, Shenzhen, China) to construct the target vector (refereed as AAV5-miR-29a) according to the manufacturer’s instruction.】

 

7. Galectin-9 ameliorates respiratory syncytial virus-induced pulmonaryimmunopathology through regulating the balance between Th17 andregulatory T cells
Xiaoxia Lua,∗,1, Karen S. McCoyb,1, Jiali Xua, Weikun Huc, Hebin Chena, Kun Jianga,Feng Hana, Peng Chena, Yanli Wanga
a.Department of Pulmonary Medicine, Wuhan Children’s Hospital, Wuhan, China
b.Department of Pediatrics, Division of Pulmonary Medicine, Nationwide Children’s Hospital and Ohio State University School of Medicine and PublicHealth, Columbus, OH, USA
c.Department of Ophthalmology, Tongi Hospital, Tongi Medical College, Huazhong University of Science and Technology, Wuhan, China
【The rAAV9-Gal-9 and rAAV9-ZsGreen viral particles were gen-erated using the HET kit following the manufacturer’s instructions(Biowit Technologies, China). 】


8. miR-134 Regulates Ischemia/Reperfusion Injury-Induced Neuronal Cell Death by Regulating CREB Signaling
Weidong Huang & Xiaobin Liu & Jie Cao & Facai Meng &Min Li & Bo Chen & Jie Zhang
【Recombinant adeno-associated virus (AAV) vectors were constructed by Biowit Technologies (Biowit, Shenzhen,China).】


9. pAAV-hSOX9-IRES-tdTomato recombinant plasmid constructs adeno-related virus package
Diao Ze-zheng, Yan Guo-qing, Zhang Zhi-wei, Fang Jing, Xu Peng, Xi Yong-ming, Ren Shan, Liu Yong-jun, Sui Ai-hua
Department of Spinal Surgery, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China

 

10. VEGF165基因重组腺相关病毒构建及转染猪骨髓间充质干细胞的研究
 夏利龙; 朱成楚; 陈保富; 张波; 陈宇; 胡炳川; 张健;
温州医科大学附属台州医院;
【1.主要试剂:细胞AAV 包装质粒pAAV-IRES-Zs-Green、JM109感受态细菌、HET转染试剂盒(深圳百恩维生物科技有限公司)】


11. 腺相关病毒载体介导ZsGreen 基因通过3 种转染途径在心肌组织转染效果的比较
谭文鹏,杨侃,李安莹,杨琼
(中南大学湘雅三医院心内科,长沙410013)
【携带报告基因ZsGreen 的腺相关病毒-9(Adeno-associated virus 9-ZsGreen,AAV9-ZsGreen)购自百恩维生物公司】


12. 同一滴度不同方法转染AAV9-SERCA2a 基因对大鼠的有效性和毒副作用评价
徐晓霞1,周祁娜1,姜涛2,王红丽2,王欢1,季萌1,王凌鹏1,张广伟1,侯月梅1
( 1. 新疆医科大学第一附属医院心律失常科,乌鲁木齐830054;2.新疆医科大学第一附属医院医学研究中心,乌鲁木齐830054)
【携带EGFP 的9 型腺相关病毒( AAV9-EGFP) 、携带SERCA2a-EGPF 的9 型腺相关病毒( AAV9-
SERCA2a-EGFP) 购自深圳市百恩维生物科技有限公司】


13. 9型重组腺相关病毒转染microRNA-21 前体在大鼠心肌的转染效率及安全性
杨琼1,杨侃1,李安莹1,谭文鹏2
(1.中南大学湘雅三医院心内科,湖南长沙410013;2.广州医学院第三附属医院心内科,广东广州510150)【rAAV9- Zs-Green-pre-miR-21 由深圳百恩维生物公司构建】